Leishmania tarentolae as a host for heterologous expression of functional human ABCB6 transporter
نویسندگان
چکیده
منابع مشابه
Leishmania tarentolae as a host for heterologous expression of functional human ABCB6 transporter.
The need for large amounts of reproducibly produced and isolated protein arises not only in structural studies, but even more so in biochemical ones, and with regard to ABC transporters it is especially pressing when faced with the prospect of enzymatic/transport activity studies, substrate screening etc. Thus, reliable heterologous expression systems/model organisms for large and complex prote...
متن کاملDevelopment of a defined medium for heterologous expression in Leishmania tarentolae.
A defined medium is essential for studying nutritional requirements of microorganisms and for selective supplementation of necessary substances. Hemin is an essential ingredient for growth of Leishmania tarentolae, but tends to precipitate in aqueous solutions without further stabilization. These aggregates disturb the measurement of the optical density or the cell density and the following dow...
متن کاملHeterologous Expression of Human IL-29 (IFN-λ1) in Iranian Lizard Leishmania
Background: Interferons with diffrent functions such as antiviral, antiproliferative and immunomodulatory actions are effctive <span style="font-varia...
متن کاملRecombinant protein expression in Leishmania tarentolae.
A variety of recombinant protein expression systems have been developed for heterologous genes in both prokaryotic and eukaryotic systems such as bacteria, yeast, mammals, insects, transgenic animals, and plants. Recently Leishmania tarentolae, a trypanosomatid protozoan parasite of the white-spotted wall gecko (Tarentola annularis), has been suggested as candidate for heterologous genes expres...
متن کاملA highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker
BACKGROUND Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, however, time consuming and require extensive lab work in order to identify well-expressing cell lines. Here we established an alternative protein expression work-flow that emplo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2016
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2016.06.022